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Addgene inc pcmvvsv g
Pcmvvsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3091 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmvvsv-g/pCMV-VSV-G+(Plasmid+%238454)/pm41786974-44-41-42
Average 96 stars, based on 3091 article reviews
pcmvvsv g - by Bioz Stars, 2026-09
96/100 stars

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Generated:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

Cotransfection:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

Cloning:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

Construct:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

Virus:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

Plasmid Preparation:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

Transfection:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

shRNA:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

Control:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.

Retroviral:

Article Title: Mechanistic Analysis of a DNA Damage-Induced, PTEN-Dependent Size Checkpoint in Human Cells
Article Snippet: The lentiviral helper plasmids pHR CMV8.2 R and pCMVVSV-G were also obtained from Addgene.

Article Title: Systematic functional characterization of non-coding regulatory SNPs associated with central obesity.
Article Snippet: ARTICLE Systematic functional characterization of non-coding regulatory SNPs associated with central obesity

Article Title: Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions
Article Snippet: Briefly, 293T cells were transfected with the lentiviral vector and the packaging plasmids, pCMV-dR8.2 and pCMVVSV-G (both obtained from Addgene) using Fugene 6.

Article Title: Identification of New Compounds against PRRSV Infection by Directly Targeting CD163.
Article Snippet: The porcine reproductive and respiratory syndrome viruses (PRRSV) led to a global panzootic and huge economical losses to the pork industry.. PRRSV targets the scavenger receptor CD163 for productive infection.. However, currently no effective treatment is available to control the spread of this disease.

Article Title: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Article Snippet: For each well, 0.5 μ g of pCMV-dR8.2 dvpr (Addgene) and 0.3 μ g of pCMVVSV-G (Addgene), each constructed to carry human immunodeficiency virus long terminal repeats, and 1.5 μ g of plasmid containing the gene of interest were cotransfected with TransIT transfection reagent (Mirus) according to the manufacturer’s instructions.

Article Title: Differential effects of Akt isoforms on somatic cell reprogramming.
Article Snippet: The vectors for pMXs-Oct4, Klf4, Sox2 and c-Myc (Takahashi and Yamanaka, 2006), pLKO.1-puro, pLKO.1-scramble shRNA control, FUW-M2rtTA and FUW-TetO-Esrrb (Buganim et al., 2012), retroviral and lentiviral packaging constructs pUMVC, pCMVVSV-G and psPAX2 (Stewart et al., 2003) were all obtained from Addgene. pLKO.1-shRNA constructs against mouse Akt1 and Akt2 were purchased from Sigma.

Article Title: Ligand-binding Domain–activating Mutations of ESR1 Rewire Cellular Metabolism of Breast Cancer Cells
Article Snippet: All subcloned constructs were sequenced. pCMVVSV-G was a gift from Bob Weinberg (Addgene plasmid catalog no. 8454; ref. 19), psPAX2 was a gift fromDidier Trono (Addgene plasmid catalog no. 12260).

Article Title: Autophagy sustains mitochondrial respiration and determines resistance to BRAF V600E inhibition in thyroid carcinoma cells.
Article Snippet: BRAF is the most prevalent mutation in thyroid cancer and correlates with poor prognosis and therapy resistance.. Although selective inhibitors of BRAF have been developed, more advanced tumors such as anaplastic thyroid carcinomas show a poor response in clinical trials.. Therefore, the study of alternative survival mechanisms is needed.



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